Aerobic Bacteria Pathogens Associated with Caprine Mastitis in Nsukka Area of Enugu State.
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Abstract
This study ascertained the aerobic bacteria associated with cases of clinical and subclinical caprine mastitis. A total of 58 lactating West African dwarf does were used for this investigation. These lactating does had signs of clinical and sub-clinical mastitis. These samples were collected from Nsukka town, Nsukka abattoir, Orba Market, Obukpa Market and Obolllo - Afor market, all in Enugu State, Nigeria. Clinical mastitis was detected by gross signs of udder infection by physical examination of abnormal milk, whereas subclinical mastitis was recognized using California Mastitis test. This study showed that 39 (67.24%) of 58 goats were positive for mastitis on California Mastitis Test. Clinical and subclinical cases of caprine mastitis, Staphylococcus aureus was the predominant pathogen 32 (55.17%). 14 (24.13%) of the isolates were positive for Staphylococcus epidermidis, 6 (10.34%) for Streptoococcus agalactiae, 4 (6.89%) E.coli and 2 (3.44%) for Klebsiella pneumoniae. This work identified the bacterial species linked with caprine mastitis in Nsukka area of Enugu State.
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Adetunji, V. O. and Olaoye, O. O (2012). Incidence and antibiotic susceptibility pattern of Listeria monocytogenes isolates from milk of West African Dwarf and Red Sokoto breeds of goat from Southwestern Nigeria. New York Sci J. 5 (11): 68-73.
Ahmadi, N., Yazdi, H.S., Derakhshandeh, A. and Ghane M. (2014). Acute Mastitis Caused by Pasteurella multocida in a goat: Clinicopathological and Microbiological Findings. Braz J Vet. Pathol; 7 (1): 25 – 28.
Ajuwape, A.T.P., Roberts, A.A., Solarin, A.A. and Adetosoye, A.I. (2005). Bacteriological and haematological studies of clinical mastitis in goats in Ibadan, Oyo State, Nigeria. Small. Rum. Res. 60: 307-310.
Ali, Z., Muhammad, G., Ahmad T, Khan, R., Naz, S., Hasib-Anwar, H., Farooq A. F., Manzoor, M.N. and Usama, AR. (2010). Prevalence of Caprine Sub-Clinical Mastitis, its Etiological Agents and their Sensitivity to Antibiotics in Indigenous Breeds of Kohat, Pakistan. Pak life Soc Sci. 8 (1): 63-67.
Ameh, J.A. and Tari, I.S. (2000). Observation on the prevalence of caprine mastitis in relation to predisposing factors in Maiduguri. Small Rum Res 35(1): 1-5
Assefa, W., Molla, B., Belihu, K.and Kleer, J. and Hildebrandt, G. (2006). A cross-sectional study on the prevalence, antimicrobial susceptibility patterns, and associated bacterial pathogens of goat mastitis. Int J Appl Res Vet Med 4: 169-176.
Bedolla, C.C. and Castañeda, V.H. (2003). Agentes patógenos causantes de la mastitis bovina. Cuatro vientos. 38: 27-29.
Bergdoll, M.S. (1989). Staphylococcus aureus. In: Doyle, M.P. (Ed) Foodborne bacterial pathogens.. New York and Basel: Marcel Dekker; Pp 463-473.
Bergonier, D., De Crémoux R., Rupp R., Lagriffoul G., Berthelot, X. (2003). Mastitis of dairy small ruminants. Vet. Res. 34: 689–716.
Bilal, M., Iqbal, M., Muhammad, G.and Avais, M. (2004).Factors Affecting the Prevalence of Clinical Mastitis in Buffaloes around Faisalabad District (Pakistan). Int J Agri Biol 6: 185-187.
Castañeda V.H, Jäger S, Wolter W, Zschöck M, Castañeda V.M.A. and El-Sayed A. (2013) Isolation and identification of main mastitis pathogens in Mexico. Arq Bras Med Vet Zootec 65(2): 377-382.
Chessbrough, M. (2000). District Laboratory Practice in Tropical Countries Part 2. Low Price Edn. Cambridge UK. p. 39-70, 395.
Contreras, A.C., Sánchez, L.A. and Corrales, J.C. (2003). The role of intramammary pathogens in dairy goats. Livest Prod Sci. 79:273-83.
Contreras, A., D., Sierra, A., Sanchez, J.C., Corrales, J.C., Marcoc, M.J. Paape and Gonzalo, C. (2007).Mastitis in small ruminants. Small Rum. Res., 68:145-153.
Cortimiglia, C., Bianchini, V., Franco, A, Caprioli A, Battisti A, Colombo, L., Stradiotto, K., Vezzoli, F. and Luini, M. (2015) Short communication: Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in bulk tank milk of dairy goat farms from Northern Italy. J
Dairy Sci 98: 2307–2311.
Fthenakis, G.C. and Jones, J. E. T. (1990). The effect of experimentally induced subclinical mastitis on milk yield of ewes and on the growth of lambs. Br Vet J. 14:43-49.
Fthenakis, G.C. (1995). Californa mastitis test and Whiteside test in diagnosis of subclinical masttis of diary ewes. Small Rum Res. 13: 293-300.
Gonzales- Rodriguez, M.C and Carmanes P. (1996). Evaluation of the California mastitis test as a discriminant method to detect subclinical mastitis in dairy cows. Int J Antimicrob Agents; 19:219-226.
Greenwood, D., Slack R.C.B and Peutherer, J.F. (2002). Medical Microbiology. 16th Edn., Churchill Livingstone, Edinburgh-London-New York-Philadelphia-St. Louis-Sydney-Toronto, pp: 168-173.
Haenlein, G.F.W. (2004). Goats milk in human nutrition. Small Rum Res, 51: 155-163.
Harmon, R.J., Eberhardt, R.J. and Jasper, D.E. (1990). Microbiological procedures for the diagnosis of bovine udder infection, 3rd edition. Arlington, VA: National Mastitis Council; p. 34.
Hawari, A.D., Obeidat, M., Saddam, S.H. Awaisheh, S.S., Al-Daghistani, H.I., Al- Abbadi, A.A., Omar, S.S., Qrunfleh, I.M. et al. (2014). Prevalence of Mastitis pathogens and their resistance against antimicrobial agents in Awassi sheep in Al-Balqa Province of Jordan. Amer J Anim Vet Sci; 9 (2): 116-121.
Ibrahim, A., Kursat ,K., Haci, A.C. (2009). Identification and antimicrobial subseptibilty of sub-clinical mastitis pathogens isolated from hair goat’s milk. J Anim Vet Adv 8 (6): 1086-1090.
Islam, M.A., Samad, M.A.and Anisur-Rahman, A.K.M. (2011). Bacterial pathogens and Risk Factors associated with Mastitis in Black Bengal goats in Bangladesh. Bangl J Vet Med 9 (2): 155 – 159.
Kateete, D.P., Kabugo, U., Baluku, H., Nyakarahuka, L., Kyobe, S., Okee, M., Najjuka, C.F.and Joloba M.L. (2013). Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda. PLoS ONE, 8(5): e63413.
Kitara, L.D., AD Anywar, A.D., Acullu, D., Odongo-Aginya, E., Aloyo, J and Fendu, M. (2011). Antibiotic susceptibility of Staphylococcus aureus in suppurative lesions in Lacor Hospital, Uganda. Afr Health Sci 11(1): S34–S39.
Lawal-Adebowale OA. (2012) Dynamics of Ruminant Livestock Management in the Context of the Nigerian Agricultural System. In: Javed K. (editor) Livestock Production. pp. 60-63.
Leitner, G., Merin, U., Silanikove, N., Ezra, E., Chaffer, M., Gollop, N., Winkler, M., Glickman, A. and Saran, A. (2004). Effect of subclinical intramammary infection on somatic cell counts, NAGase activity and gross composition of goats' milk. J Dairy Res 71(3): 311-315.
Maisi, P. and Riipinnen I. (1988). Use of California mastitis test, N-acetyl-glucosamidase and antitrypsin to diagnose caprine subclinical mastitis. J Dairy Res 55: 309-314.
Maisi, P. (1990). Analysis of physiological changes in caprine milk with CMT, NA Gase and antitrypsin. Small Rum Res; 3: 485-492.
Mbindyo, C.M., Gitao, C.G.and Bebora, L. (2014). A cross-sectional study on the prevalence of subclinical mastitis and antimicrobial susceptibility patterns of the bacterial isolates in milk samples of smallholder dairy goats in Kenya. Amer J Res Commun. 2(8): 30-51.
Mørk, T., Waage, S., Tollersrud, T., Kvitle, B. and Sviland, S., (2007). Clinical mastitis in ewes; bacteriology, epidemiology and clinical features. Acta Vet Scand 49: (23): 1-8.
Ndegwa, E.N., Mulei, C.M. and Munyua, S.J.M. (2001). Prevalence of microorganisms associated with udder infections in dairy goats on small-scale farms in Kenya. J S Afr Vet Ass, 72 (2): 97–98.
Radostitis, O. M., Gay, C. C., Blood, D. C. and Hinchcliff, K. W. (2000). Mastitis. In Veterinary Medicine 9th ed., W. B. Saunders, London. pp. 601-700.
Razi, K.M.A, Rahman, M.B., Flores-Gutiérrez, G.H. and Rahman, M.T. (2012). Prevalence of caprine subclinical mastitis in Mymensingh area and characterization of associated bacterial agents and the risk factors. Microb. Hlth 1 (1): 1-5.
Sanchez, A., Contreras, A., Corrales, J.C. and Munoz, P. (2004). Influence of sampling time on bacteriological diagnosis of goat intramammary infection. Vet Microbiol 98: 329-332.
Sousa, M.A., Parente, C.E.S.R., Motta, O.V., Bonna, I.C.F., Silva, D.A. and Lencastre, H. (2007). Characterization of Staphylococcus aureus isolates from buffalo, bovine, ovine, and caprine milk samples collected in Rio de Janeiro State, Brazil. Appl Envir Microbiol 73 (12): 3845–3849.
Togun, V.A., Amao, O.A., Oyebode, O.A. and Ajibade, E.A. (2003). Occurrence of Mastitiscases in West African dwarf goats under extensive system of management in Ogbomoso. Asset Series A. 3 (4):121-125.
Tollersrud, T., Kenny, K., Caugant, D. A. and Lund, A. (2000). Characterisation of isolates of Staphylococcus aureus from acute, chronic and subclinical mastitis in cows in Norway. APMIS 108: 565-572.
Ugochukwu, E.I. (1983a). Staphylococccal mastitis in West African dwarf goats. A case report. Nig Vet J. 12 (19): 9-12.
Ugochukwu, E.I., (1983b). Isolation, identification and antibiotic sensitivity of Staphylococcus spp from goats suffering from mastitis in Anambra State of Nigeria. Bull Anim Hlth Prod Africa 1983b; 31(4): 349-352.
Ugochukwu, E.I. (2008). Isolation and characterization of Pasteurella multocida from caprine pneumonic lungs. Anim Res Int 5 (2): 880-882.
Virdis, S., Scarano, C., Cossu, F., Spanu, V., Spanu, C.and De Santis, E.P.L. (2010). Antibiotic resistance in Staphylococcus aureus and Coagulase NegativeStaphylococci isolated from goats with subclinical mastitis. Vet Med Int. Article ID 517060.
Wolter, W., Castaneda, H., Klopert, B. and Zchöck, M. (2004). Mastitis Bovina, Prevención, Diagnostico y Tratamiento. 1. Ed. Editorial Universitaria: Universidad de Guadalajara. Guadalajara Jalisco. p.1-146.
Yousof-Beighi, G.H., Rad, M., Hosseini, S.M. and Jafari A. (2005). Effect of centrifugation of milk samples on bacteriologic culture. Iranian J. Vet Res 6: 59-61.